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Tissue CultureAstrocytes from postnatal SD rat at day 1 were isolated and cultured. KXS Decoction PreparationKXS decoction was composed of the following dried raw materials: Ginseng Radix et Rhizome (root online loans rhizome of P. KXS Stimulates the Expression of Neurotrophic Factors on AstrocytesCultured astrocytes reached confluence on the day in vitro (DIV) 12 having the mRNA level of glial fibrillary acidic protein (GFAP), a marker for astrocyte, reaching the online loans on DIV 12 to 16 (Figure 1(a)).


Figure 1: Cultures of rat astrocytes. Figure 2: KXS stimulates the mRNA expression of neurotrophic factors in astrocytes.


The mRNA expression levels of neurotrophic factors (NGF, GDNF, BDNF, NT3, NT4, and NT5) in astrocytes were analyzed. The astrocytes were treated with different formulations of KXS (0. The mRNA was determined by quantitative PCR.


Primers for neurotrophic factors were listed in Supplementary Table 1. Figure 3: KXS stimulates the secretion of neurotrophic factor online loans in astrocytes.


The calibration curve of each target protein was constructed by plotting absorbance versus the concentration of each target protein. Each calibration curve was derived from 5 data points. Then, the media were collected for analysis of NGF, BDNF, and GDNF with ELISA kit. Data are expressed as percentage of increase to control (no drug treatment, that is, 0 extract here). Figure 4: The comparison of KXS and single herb in stimulating the secretion of neurotrophic factors in astrocytes.


Figure 5: Effect of cAMP, MAPK, and estrogen signaling pathway on the expression of neurotrophic factors. The mRNA expression levels of neurotrophic factors in astrocytes were analyzed. Windhorst, Encyclopedia of Neuroscience, Springer, Berlin, Germany, 2009. View at Google ScholarK. View at Google ScholarY. View at Google ScholarZ. Response Time Response Rate 85. Xin Kai SR-V X3. Neben der Great Wall Motor Company ist auf dem Pariser Automobilsalon noch online loans ein weiterer chinesischer Hersteller vertreten.